[1]汤安群,江涛,白津.KIF4A在结直肠癌细胞增殖及周期中的作用及其机制[J].徐州医科大学学报,2020,40(11):781-785.[doi:10.3969/j.issn.2096-3882.2020.11.001]
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KIF4A在结直肠癌细胞增殖及周期中的作用及其机制()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
40
期数:
2020年11期
页码:
781-785
栏目:
出版日期:
2020-11-25

文章信息/Info

Title:
The role and mechanism of KIF4A in the proliferation and cell cycle of colorectal cancer cells
作者:
汤安群 江涛 白津
徐州医科大学肿瘤防治研究所,江苏 徐州 221002;徐州医科大学附属医院普外科,江苏 徐州 221002
关键词:
结直肠癌 增殖 周期 KIF4A
DOI:
10.3969/j.issn.2096-3882.2020.11.001
文献标志码:
A
摘要:
目的 探讨在结直肠癌细胞中沉默KIF4A基因后,对细胞增殖及周期的影响以及相关机制.方法 分别使用KIF4A-CtrlRNA(si-Ctrl)及KIF4A-siRNA(si-KIF4A)瞬时转染结直肠癌细胞株SW480和DLD1,通过Western blot验证干扰效果;CCK8实验检测干扰KIF4A后SW480和DLD1细胞的增殖能力;流式细胞术检测干扰KIF4A后对细胞周期的影响;Western blot检测周期相关蛋白Cyclin D1、Cyclin E2和CDK2表达水平的变化.结果 si-KIF4A组较si-Ctrl组细胞增殖能力显著下降;细胞周期出现G0/G1周期阻滞;周期相关蛋白Cyclin D1、Cyclin E2和CDK2的蛋白表达水平下降.结论 KIF4A通过上调周期蛋白Cyclin D1、Cyclin E2和CDK2的表达促进结直肠癌细胞的增殖.
Abstract:
ob<x>jective To investigate the effect and relate d mechanism of KIF4A on cell proliferation and cell cycle of colorectal cancer cells. Methods The KIF4A-CtrlRNA ( si-Ctrl) and KIF4A-siRNA ( si-KIF4A) were transiently transfect ed into colorectal cancer cell lines SW480 and DLD1, Western b lot was used to verified the effect of knocking down; the changes of cell p roliferation of SW480 and DLD1 colorectal cells were confirmed by CCK8 assay; flow cytometry was performed to analyze the effect of KIF4A on cell cycle; the changes of cell cycle-related proteins Cyclin D1, Cyclin E2 and CDK2 were performed by Western blot. Results Comparing the si-KIF4A group with si-Ctrl group, the ex<x>pression of KIF4A was significantly reduced; the cell proliferation was dramatically decreased; the cell cycle was arrested at G0/G1 phase and the cell cycle-related proteins Cyclin D1, Cyclin E2 and CDK2 were downregulated. Conclusion KIF4A promotes the cell proliferation via upregulating the ex<x>pression of Cyclin D1, Cyclin E2 and CDK2

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更新日期/Last Update: 2020-12-15