[1]陆峥,程乾,柴大飞,等.白细胞介素-24基因联合三氧化二砷促进肾癌Ketr-3细胞凋亡[J].徐州医科大学学报,2021,41(03):157-160.[doi:10.3969/j.issn.2096-3882.2021.03.001]
 Interleukin-24 gene combined with arsenic trioxide promotes apoptosis on kidney cancer Ketr-3 cells[J].Journal of Xuzhou Medical University,2021,41(03):157-160.[doi:10.3969/j.issn.2096-3882.2021.03.001]
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白细胞介素-24基因联合三氧化二砷促进肾癌Ketr-3细胞凋亡()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
41
期数:
2021年03期
页码:
157-160
栏目:
出版日期:
2021-03-25

文章信息/Info

Title:
Interleukin-24 gene combined with arsenic trioxide promotes apoptosis on kidney cancer Ketr-3 cells
作者:
陆峥程乾柴大飞郑骏年*
徐州医科大学肿瘤防治研究所,江苏徐州221002
关键词:
白细胞介素24三氧化二砷肾癌细胞
分类号:
R737.14
DOI:
10.3969/j.issn.2096-3882.2021.03.001
文献标志码:
A
摘要:
目的观察白细胞介素-24基因(IL-24)联合三氧化二砷(As2O3)对肾癌Ketr-3细胞的杀伤作用。方法细胞传代后分为4组:PBS组(磷酸盐缓冲液)、As2O3组(As2O3 5 mg/L)、IL-24组(pCDNA3.1-IL-24 3 μg)、联用组:(pCDNA3.1-IL-24=3 μg+As2O3=5 mg/L),处理细胞48 h后进行检测。酶联免疫吸附实验(ELISA)检测4组细胞IL-24蛋白分泌表达的变化;单细胞凝胶电泳实验观察4组细胞的DNA断裂情况;微核实验观察4组细胞染色体断裂情况;流式细胞术检测4组细胞的凋亡情况。结果ELISA结果显示:与PBS组比较,IL-24组和联用组IL-24表达量明显升高,差异有统计学意义(P<0.01);单细胞凝胶电泳、微核实验显示:与As2O3组相比,联用组DNA与染色体损伤增加,差异有统计学意义(P<0.05)。流式细胞术显示:与PBS组相比,As2O3组、IL-24组、联用组的细胞凋亡率显著增强(P<0.05),其中联用组凋亡率增强更为明显(P<0.05),差异均有统计学意义。结论IL-24联合As2O3有协同作用,可进一步增强对肾癌Ketr-3细胞的杀伤作用。
Abstract:
objective To investigate the effect of interleukin-24 gene (IL-24) combined with As2O3 on renal cancer cell Ketr-3. Methods Ketr-3 cells were seeded onto 6-well plates and divided into 4 groups, PBS group (phosphate buffer saline), As2O3 group(As2O3=5μg?mL-1), IL-24 group(pCDNA3.1-IL-24=3 μg), and combination group(pCDNA3.1-IL-24=3 μg+ As2O3=5μg?mL-1). 48 h later, the following assays were performed. The expression of IL-24 protein in the 4 treatment groups was detected by enzyme-linked immunosorbent assay (ELISA). Single cell gel electrophoresis was used to observe the DNA fragmentation. Micronucleus experiment was carried out to detect the cellular chromosome fracture. Flow cytometry was used to detect the cell apoptosis. Results The ELISA assay results showed that the expression of IL-24 were 12.29±4.39 pg?mL-1, 13.93±3.69 pg?mL-1, 332.21±53.12 pg?mL-1 and 339.56±62.31 pg?mL-1 in PBS group, As2O3 group, IL-24 group and the combination group respectively. Compared with PBS group, the expression of IL-24 in IL-24 group and the combined group increased significantly, and the difference was statistically significant (P<0.01). Single cell gel electrophoresis and micronucleus test showed that DNA and chromosome damage increased in the combination group, comparing with As2O3 group, the difference was statistically significant (P<0.05). Flow cytometry assay showed that the apoptosis rate (%) in As2O3 group, IL-24 group and combination group enhanced significantly compared with PBS group(23.47±5.15% vs 1.81±0.26%, P<0.05; 15.06±3.69% vs 1.81±0.26%, P<0.05; 28.98±5.69% vs 1.81±0.26%, P<0.05). Comparing with As2O3 group and IL-24 group, the apoptotic rate of the combination group was much higher, and the difference was statistically significant(23.47±5.15% vs 28.98±5.69%, P<0.05; 15.06±3.69% vs 28.98±5.69%, P<0.05). Conclusion The IL-24 combined with As2O3 had synergistic effect and can further enhance the antitumor effect on renal cancer cell Ketr-3.
更新日期/Last Update: 2021-04-25