[1]周正蔚,徐敏,张倪,等.臭椿酮对黑色素瘤细胞侵袭转移的抑制作用[J].徐州医科大学学报,2021,41(03):173-178.[doi:10.3969/j.issn.2096-3882.2021.03.004]
 Inhibitory effects of ailanthone on the invasion and metastasis of melanoma cells[J].Journal of Xuzhou Medical University,2021,41(03):173-178.[doi:10.3969/j.issn.2096-3882.2021.03.004]
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臭椿酮对黑色素瘤细胞侵袭转移的抑制作用()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
41
期数:
2021年03期
页码:
173-178
栏目:
出版日期:
2021-03-25

文章信息/Info

Title:
Inhibitory effects of ailanthone on the invasion and metastasis of melanoma cells
作者:
周正蔚徐敏张倪王磊刘国翔李成林*
徐州医科大学江苏省新药研究与临床药学重点实验室,江苏徐州221004
关键词:
黑色素瘤侵袭转移臭椿酮
分类号:
R962
DOI:
10.3969/j.issn.2096-3882.2021.03.004
文献标志码:
A
摘要:
目的研究臭椿酮对黑色素瘤细胞侵袭转移的影响。方法CCK-8实验检测臭椿酮对人黑色素瘤A375细胞活力的影响;侵袭实验评估臭椿酮对A375细胞侵袭能力的影响;Western blot实验考察基质金属蛋白酶MMP-2和MMP-9表达变化;小鼠黑色素瘤B16-F10细胞肺转移模型考察臭椿酮在体抑制黑色素瘤转移和对主要脏器的影响;免疫组织化学染色检测肺转移结节中MMP-2和MMP-9的表达。结果臭椿酮可抑制A375细胞活力;侵袭实验结果显示臭椿酮显著抑制A375细胞侵袭,同时抑制MMP-2和MMP-9的表达;臭椿酮显著降低肺转移结节数,并抑制MMP-2和MMP-9的表达,但对小鼠体重和重要脏器无明显影响。结论臭椿酮可抑制黑色素瘤细胞侵袭转移。
Abstract:
ObjectiveTo investigate the effects of ailanthone on the invasion and metastasis of melanoma cells.MethodsCCK-8 assay was used to detect the effects of ailanthone on the growth of human melanoma A375 cells. Invasion assay was employed to test the effects of ailanthone on the invasion of A375 cells.The expression of MMP-2 and MMP-9 was determined by Western blot. A lung metastatic model was established using murine melanoma B16-F10 cells to determine the anti-metastatic effects and toxic effects of ailanthone in vivo. Immunohistochemistry assay was used to detect the expression of MMP-2 and MMP-9 in the metastatic nodules.ResultsThe growth of A375 was inhibited after ailanthone treatment. Furthermore, ailanthone significantly inhibited the invasion of A375 cells and reduced the expression of MMP-2 and MMP-9.Ailanthone also relieve the lung metastasis of B16F10 murine melanoma cells in vivo. However, no significant weight loss nor histopathological abnormalities was observed in the major organs of mice after ailanthone treatment.ConclusionsAilanthone inhibits the invasion and metastasis of melanoma cells.
更新日期/Last Update: 2021-04-25