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    周京宇, 高红, 孙旭东, 黄小雨, 江涛, 宋军, 王建. CUX1对结直肠癌细胞增殖和转移的影响及机制研究[J]. 徐州医科大学学报, 2021, 41(11): 787-791. DOI: 10.3969/j.issn.2096-3882.2021.11.002
    引用本文: 周京宇, 高红, 孙旭东, 黄小雨, 江涛, 宋军, 王建. CUX1对结直肠癌细胞增殖和转移的影响及机制研究[J]. 徐州医科大学学报, 2021, 41(11): 787-791. DOI: 10.3969/j.issn.2096-3882.2021.11.002
    Effects of CUX1 on the profiferation and me<x>tastasis of colorectal cancer cells and its mechanism[J]. Journal of Xuzhou Medical University, 2021, 41(11): 787-791. DOI: 10.3969/j.issn.2096-3882.2021.11.002
    Citation: Effects of CUX1 on the profiferation and me<x>tastasis of colorectal cancer cells and its mechanism[J]. Journal of Xuzhou Medical University, 2021, 41(11): 787-791. DOI: 10.3969/j.issn.2096-3882.2021.11.002

    CUX1对结直肠癌细胞增殖和转移的影响及机制研究

    Effects of CUX1 on the profiferation and me<x>tastasis of colorectal cancer cells and its mechanism

    • 摘要: 目的探讨CCUT样同源盒1(CUX1)对结直肠癌细胞增殖与转移的影响及机制。方法利用TCGA数据库分析结直肠癌患者的癌组织和癌旁组织中CUX1的表达情况,通过实时荧光定量PCR(qRT-PCR)检测CUX1在结直肠癌组织中的表达情况;利用 GEPIA数据库分析CUX1表达对结直肠癌患者术后总体生存率的影响;利用siRNA-NC和siRNA-CUX1转染人结直肠癌细胞;CCK-8、Transwell实验检测CUX1对结直肠癌细胞增殖与转移能力的影响;免疫印迹检测CUX1的干扰效率以及CUX1、E-cadherin、Vimentin、N-cadherin蛋白的表达情况。结果TCGA数据库及qRT-PCR结果显示CUX1在结直肠癌组织中高表达(P<0.05);GEPIA数据库结果显示CUX1低表达组的总体生存率明显优于高表达组(P<0.01);Transwell实验结果显示siRNA-CUX1降低结直肠癌细胞的转移能力(P<0.001);CCK-8实验结果显示siRNA-CUX1抑制结直肠癌细胞的增殖能力(P<0.001);免疫印迹检测结果显示转染siRNA-CUX1后结直肠癌细胞的CUX1、N-cadherin及Vimentin蛋白表达降低,E-cadherin蛋白表达升高 (P<0.05)。结论干扰CUX1对结直肠癌细胞的增殖和转移能力有抑制作用。

       

      Abstract: ob<x>jectiveTo investigate the effect of CUX1 gene on the proliferation and me<x>tastasis of colorectal canc er.Methods The ex<x>pression of CUX1 in cancer and adjacent tissues of colorectal cancer patients was analyzed by using TCGA databa<x>se, and the ex<x>pression of CUX1 in colorectal cancer tissues was detected by real-time fluorescence quantitative PCR( qRT-PCR).The GEPIA databa<x>se was used to analyze the effect of CUX1 ex<x>pression on the overall survival rate of patients with colorectal cancer after surgery. siRNA-Ctrl and siRNA-CUX1 were transfected into human colon cancer cells.C CK-8 and Transwell assay were used to detect the effect of CUX1 on the proliferation and me<x>tastasis of colorectal cancer cells.The interference efficiency of CUX1 and the ex<x>pression of E-cadherin, Vimentin and N-cadherin proteins were detected by western blotting.Results The results of TCGA databa<x>se and qRT-PCR showed that CUX1 was highly expressed in colorectal cancer tissues (P<0.05).The results of GEPIA databa<x>se showed that the overall survival rate of the low ex<x>pression group was significantly better than that of the high ex<x>pression group (P<0.01).The results of Transwell assay showed that siRNA- CUX1 reduced the me<x>tastatic ability of colorectal cancer cells.CCK-8 assay showed that siRNA- CUX1 inhibited the proliferation of colorectal cancer cells (P<0.001).Western blot results showed that the ex<x>pression of CUX1, N-cadherin and Vimentin in colorectal cancer cells were decreased after siRNA- CUX1 transfection.The ex<x>pression of E-cadherin was increased (P<0.05). Conclusion Knockdown of CUX1 can inhibit the proliferation and me<x>tastasis of colorectal cancer.

       

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