Abstract: ob＜x＞jective The purpose of this study is to determine the significance of restoration of NLR family CARD domain-containing protein 4 (NLRC4) expressed in acute cerebral infarction (ACI) . Methods 39 cases patients with ACI and 40 healthy controls in our hospital from May 201 9 to August 20 20 were selected as the subjects of the study. The ex＜x＞pression levels of NLRC4, IL-1β and IL-18 in peripheral blood were detected by ELISA. Then, we constructed ACI animal model by blocking the blood flow of middle cerebral artery for 24 hours in rats. Immunofluorescence staining and Western blot were used to detect the ex＜x＞pression of NLRC4 in ischemic and sham operated brain tissue. Results Compared with the control group, the ACI group showed no statistical difference in age, gender, body mass index and smoking (P＞0.05). Blood biochemical tests showed that compared with the control group, C-reactive protein in ACI patients was significantly increased, and high-density lipoprotein was reduced (P＜0.05). ELISA results showed that the levels of NLRC4 and IL-1β in the peripheral blood of ACI patients were significantly up-regulated (P＜0.05). Western blot and immunofluorescence staining confirmed that the ex＜x＞pression of NLRC4 in ischemic-injured neurons increased at the animal level. In addition, the ex＜x＞pression levels of NLRC4, IL-1β and IL-18 in the peripheral blood of ACI rats are higher than those of control rats (P＜0.05). Conclusion NLRC4 is closely related to the occurrence and development of ACI. These discoveries identify the NLRC4 inflammasomes as potential therapeutic targets for stroke