Abstract:
ob<x>jective:This study aimed to observe the effects of agmatine on neuroinflammation and cognitive function after anesthesia and surgery in adult rats.Methods:Sprague-Dawley rats were randomly divided into five groups, NS group, NS+anesthesia and surgery group(NS+Sur),low-dose,medium-dose and high-dose agmatine+anesthesia and surgery group (AGM20mg/kg+Sur,AGM40mg/kg+Sur,AGM80mg/kg+Sur).Each group underwent splenectomy with sevoflurane anesthesia, and learning and memory abilities were tested by Morris water maze.The ex<x>pression of TNF-α, IL-1β and IL-6 in the hippocampus were detected by ELISA on postoperative day 3.We used western blot to detect the ex<x>pression of the ex<x>pression of p-NF-κB p65 in the hippocampus and the ZO-1 and Occ in the brain microvascular. The ex<x>pression of GFAP in hippocampal astrocytes was detected by immunofluorescence.The permeability of blood-brain barrier in rats was detected by Evans blue.Results:Three days after anesthesia, the cognitive function of NS+Sur group was significantly decreased compared with that of NS group (P<0.01). The ex<x>pression levels of p-NF -κB P65, IL-1β, IL-6, TNF-α and GFAP in the hippocampus of NS+Sur group were significantly increased, the ex<x>pression levels of ZO-1 and Occ in brain microvessels were significantly decreased, and the exudation of Evans blue in brain tissue was significantly increased (P<0.01). Compared with NS+Sur group, the cognitive function of AGM80+Sur group was improved (P<0.05, P<0.01); the ex<x>pression levels of p-NF -κB P65, IL-1β, IL-6, TNF-α and GFAP in hippocampus of AGM80+Sur group were significantly decreased, the ex<x>pression levels of ZO-1 and Occ in brain microvessels were significantly increased, and the exudation of Evans blue in brain tissue was significantly decreased (P<0.01).Conclusions:Agmatine can reduce central nervous system inflammation, blood-brain barrier permeability and cognitive dysfunction after anesthesia and surgery in SD rats.