[1]朱苏月,沈男,周永英,等.EV71型手足口病的肠道菌群结构特征分析[J].徐州医科大学学报,2022,42(05):353-357.[doi:10.3969/j.issn.2096-3882.2022.05.008]
 Analysis of the structural characteristics of intestinal flora in EV71 hand-foot-mouth disease[J].Journal of Xuzhou Medical University,2022,42(05):353-357.[doi:10.3969/j.issn.2096-3882.2022.05.008]
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EV71型手足口病的肠道菌群结构特征分析()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
42
期数:
2022年05期
页码:
353-357
栏目:
出版日期:
2022-05-25

文章信息/Info

Title:
Analysis of the structural characteristics of intestinal flora in EV71 hand-foot-mouth disease
作者:
朱苏月沈男周永英岑曼乔继冰蒋亚洲*
徐州医科大学附属宿迁医院,南京鼓楼医院集团宿迁医院儿科,江苏宿迁223800
关键词:
EV71 手足口病 肠道菌群
分类号:
EV71
DOI:
10.3969/j.issn.2096-3882.2022.05.008
文献标志码:
A
摘要:
目的测定肠道病毒71型(EV71)型手足口病的肠道菌群结构并分析其特征。方法留取2020年1月—2021年12月经徐州医科大学附属宿迁医院确诊的EV71型手足口病及健康儿童粪便样本,提取DNA后行16S rDNA 扩增子测序,并对测序后的生物信息进行分类、量化和对比。选用α多样性分析2组间的丰度、多样性差异;选用β多样性分析2组间的菌群结构差异;选用LDA分析和LEfSe分析比较2组间的显著差异菌属。结果共收集EV71型手足口病患儿(EV71组,45例)和健康儿童(对照组,35例)粪便标本80例。相对于对照组,EV71组肠道菌群多样性(shannon指数,P>0.05)和丰度(ace指数,P>0.05)变化差异无统计学意义,但其结构呈显著改变(weighted_unifrac,P<0.05),主要表现为奈瑟菌属(P<0.05)和克雷伯菌属(P<0.01)丰度升高,而罗姆布茨菌属(P<0.01)、小杆菌属(P<0.01)以及梭菌属(P<0.05)丰度降低。结论EV71型手足口病肠道菌群紊乱,以丁酸盐产生菌属丰度降低、机会致病菌属丰度升高为特征。该结果为后期EV71型手足口病的发病机制及微生态治疗提供了部分理论基础。
Abstract:
ob<x>jective To determine the structure and characteristics of intestinal flora in EV71 hand-foot-mouth disease. Methods From January 2020 to December 2021, fecal samples of EV71 hand-foot-mouth disease and healthy children diagnosed in Suqian Hospital Affiliated to Xuzhou Medical University were collected. After extracting DNA from feces, 16S rDNA amplicon sequencing was performed, and the obtained biological information was classified, quantified and compared. α-diversity was used to analyze the differences in abundance and diversity between the two groups. β-diversity was used to analyze the differences in bacterial community structure between the two groups. LDA analysis and LEfSe analysis were used to compare the significantly different genera between the two groups. Results A total of 80 fecal samples were collected from children with EV71 hand -foot-mouth disease (EV71 group, 45 cases) and healthy children (control group, 35 cases). Compared with the control group, there were no significant changes in the diversity (shannon index, P>0.05) and abundance (ace index, P>0.05) of intestinal flora in the EV71 group . However, the structure of intestinal flora in EV71 group was significantly changed (weighted_unifrac, P<0.05), which was mainly manifested in the increase of the abundance of Neisseria ( P<0.05) and Klebsiella ( P<0.01), while the abundance of Romboutsia ( P<0.01), Dialister (P<0.01) and Clostridium ( P<0.05) were decreased . Conclusion The intestinal flora of EV71 hand-foot-mouth disease is disordered, which is mainly characterized by the decrease of butyrate producing bacteria and the increase of opportunistic pathogenic bacteria. The results provide some theoretical basis for the pathogenesis and microecological treatment of EV71 hand-foot-mouth disease in the later stage.

备注/Memo

备注/Memo:
基金项目:江苏省妇幼健康科研项目(F201941);宿迁市科技计划项目(Z2019154);徐州医科大学附属医院科技发展基金(XYFM2020017)*通信作者,E-mail:xzwaldo@163.com
更新日期/Last Update: 2022-06-07