Abstract: Objective To investigate the effect of miR-30e-5p targeting and regulating glucose-regulated protein 78 (GRP78) on the biological behavior of cervical squamous carcinoma cells SiHa.Methods Normal cervical tissues were collected from 40 subjects and cervical squamous carcinoma tissues from 60 patients. The expression of GRP78 was detected by immunohistochemistry. The expression of miR-30e-5p and GRP78 mRNA in different cervical tissues of the two groups was detected by real-time fluorescence quantitative PCR (RT-qPCR). Their relationship with clinicopathological features was analyzed. The upstream miRNA of GRP78 was predicted by bioinformation websites, and dual luciferase reporter gene assay was performed to verify their targeting relationship. MiR-30e-5p was overexpressed, and the mRNA and protein expression of GRP78 in SiHa cells were detected by RT-qPCR and Western Blot. The proliferation, migration and invasion abilities of the cells were detected by CCK8 assay, wound healing assay and Transwell assay.Results Immunohistochemistry results showed that the positive rate of GRP78 protein in cervical squamous carcinoma tissues was significantly higher than in normal cervical tissues (P<0.05). RT-qPCR results indicated that compared with normal cervical tissues, the expression of miR-30e-5p in cervical squamous carcinoma tissues was reduced, while the expression of GRP78 was elevated (P<0.05). The levels of miR-30e-5p and GRP78 mRNA in cervical squamous carcinoma tissues were significantly correlated with the degree of differentiation and lymph node metastasis, in which the expression level of miR-30e-5p was also correlated with vascular infiltration. Dual luciferase reporter gene assay confirmed a targeting relationship between miR-30e-5p and GRP78. Overexpression of miR-30e-5p in SiHa cells resulted in decreases in the mRNA and protein expression of GRP78. Cell function experiments showed that the proliferation, migration and invasion abilities of SiHa cells were inhibited to various degrees after overexpression of miR-30e-5p.Conclusions MiR-30e-5p is lowly expressed in cervical squamous carcinoma tissues, while GRP78 is highly expressed. Both of them are significantly correlated with the degree of differentiation and lymph node metastasis. MiR-30e-5p has a targeting relationship with GRP78, and overexpression of miR-30e-5p results in down-regulated GRP78 expression, thereby inhibiting the proliferation, migration, and invasive abilities of cervical squamous carcinoma cells.