Abstract: Objective To investigate the effects of drynachromoside B (DC-B), a chromone component from Rhizoma Drynariae, on the proliferation and osteogenic differentiation of mouse embryonic preosteoblasts (MC3T3-E1), and to analyze its influence on cell adhesion to titanium surfaces. Methods MC3T3-E1 cells were divided into four groups: control, 5 μmol/L DC-B, 10 μmol/L DC-B, and 20 μmol/L DC-B. Cell proliferation was evaluated using the CCK-8 assay; osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity and alizarin red staining. Western blot was used to detect the expression of Runt-related transcription factor 2 (RUNX2) and proteins related to the Wnt/β-catenin signaling pathway (β-catenin and LEF1). Cell adhesion morphology was observed through immunofluorescence staining, and cell morphology and adhesion on titanium sheets were examined by scanning electron microscopy (SEM). Results CCK-8, ALP activity, and alizarin red staining results all showed that DC-B significantly promoted the proliferation and osteogenic differentiation of MC3T3-E1 cells, with the most pronounced effect at 10 μmol/L. Compared with the control group, 10 μmol/L DC-B significantly upregulated the expression of β-catenin, LEF1, and RUNX2 (P<0.05). According to immunofluorescence staining and SEM observation, DC-B enhanced cell attachment and adhesion, particularly on titanium surfaces. Conclusions DC-B from Rhizoma Drynariae promotes the proliferation and osteogenic differentiation of MC3T3-E1 cells and enhances their adhesion to titanium surfaces. These findings provide experimental evidence and theoretical support for improving bone density and quality at implant sites through pharmacological intervention, thereby facilitating osseointegration