Abstract: Objective To investigate the expression of NKD1 in cervical cancer tissues, its effects on the proliferation and migration of cervical cancer cells, and the underlying molecular mechanisms. Methods Cervical tissue specimens were obtained through surgical resection at the Affiliated Hospital of Xuzhou Medical University. The expression of NKD1 in cervical tissue specimens was detected by real-time quantitative PCR (RT-qPCR). HeLa cervical cancer cells were transfected with plasmids to construct a stable NKD1 overexpression cell line. Cell proliferation and migration capacities were assessed by CCK-8 assay and wound healing assay. The expression of β-catenin and GSK3β was measured by Western blot. Results The expression of NKD1 in cervical cancer tissues was significantly lower than that in normal cervical tissues (P<0.05). After NKD1 overexpression, the proliferation and migration capabilities of HeLa cells were significantly reduced (P<0.05), and the levels of GSK3β and β-catenin were also significantly decreased. Conclusions NKD1 is lowly expressed in cervical cancer tissues. NKD1 overexpression significantly inhibits the proliferation and migration of HeLa cells, which may be related to the regulation of the Wnt/β-catenin signaling pathway.