Abstract: Objective To investigate the clinical features and diagnosis of myasthenia gravis（MG）.Methods The clinical data of 52 patients with MG admitted in the Department of Neurology,the Affiliated Hospital of Xuzhou Medical University from January 2015 to July 2018 were retrospectively analyzed. Results The main clinical manifestations were blepharoptosis, diplopia,hoarseness or dysphagia,limb weakness and rarely dyspnea.The positive rate of neostigmine test was 100%（52/52）.The positive rate of repetitive nerve stimulation was 90.7%（39/43）.The positive rate of thymus CT examination was 52%（26/50）.The positive rate of abnormal thyroid function was 28.9%（13/45）,of which10.5%（2/19）in males and 42.3%（11/26）in females. Conclusions MG is more common in women,mostly with ocular muscle symptom as first symptom.Types Ⅰ and Ⅱ B are more common.The positive rates of neostigmine test and repetitive electrical stimulation are higher.Women are often accompanied with thymus and thyroid dysfunction.The detection of autoantibodies is helpful to guide rational diagnosis and treatment and to evaluate prognosis.