Abstract: Objective To discuss the clinical analysis of mouse nerve growth factor combined with methylprednisolone in the treatment of sudden deafness (SD). Methods A total of 78 SD patients were randomly divided into control group and observation group, 39 cases in each group. The control group was given vasodilation, neurotrophic therapy and postaurical injection of methylprednisolone, while the observation group was given postaurical injection of mouse nerve growth factor. The serum levels of vascular endothelial cell cadherin (VE-cadherin), soluble vascular cell adhesion molecule-1 (sVCAM-1), high mobility group box-1 (HMGB1), pure tone auditory threshold and hearing impairment score were compared before and after treatment, and the time of symptom subsidence was recorded. After 2 weeks of treatment, comprehensive clinical efficacy was evaluated and adverse reactions were recorded. Results The serum levels of VE-cadherin, sVCAM-1 and HMGB1 in the two groups were significantly decreased after treatment, and the serum levels of VE-cadherin, sVCAM-1 and HMGB1 in the observation group were significantly lower than those in the control group (P<0.05). After treatment, the pure tone hearing threshold and hearing impairment score of the two groups were significantly reduced, and the pure tone hearing threshold and hearing impairment score of the observation group were significantly lower than those of the control group (P<0.05). The recovery time of tinnitus, vertigo and tightness in the observation group was significantly shorter than that in the control group (P<0.05). The total effective rate of the observation group was higher than that of the control group (92.31% vs. 82.05%, P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusions Mouse nerve growth factor combined with methylprednisolone can significantly improve the clinical symptoms of tinnitus and vertigo in SD patients, improve the hearing level, reduce the level of serum factors related to the disease, and the treatment is safe and effective.