Abstract: Objective To explore the clinical effect of daily treatment checklist combined with WeChat in the designated isolation ward of coronavirus disease 2019(COVID-19). Methods This study adopted the method of self comparison. In this study, 54 nurses from the Sixth Ward (the former Breast Department) in East Campus of Renmin Hospital of Wuhan University from February 6 to February 15, 2020 were selected as the subjects. From February 6 to February 8, 89 patients were cared for and treated in the pre-use group according to the functional nursing mode. From February 9 to February 15, 255 patients were cared for and treated in the post-use group according to the daily treatment checklist, and the holistic responsibility nursing was implemented for the patients, and the WeChat app was used to transmit the new medical order information. The treatment defects, the implementation of new orders and the satisfaction of medical staff were compared between the two groups. Results Compared with the pre-use group, the proportion of patients with treatment defects and the proportion of treatment defects in the post-use group were significantly lower, the correct rate of new doctor's order delivery was higher, and the satisfaction of medical staff was higher, the difference was statistically significant (P＜0.05). Conclusions The application of daily treatment checklist combined with WeChat in COVID-19 designated isolation ward can reduce nursing defects, facilitate the transmission of doctor's orders, improve nurses' work efficiency, reduce psychological burden and improve the satisfaction of medical staff.