Correlation between B/E value in the intestinal flora and serum Gd-IgA1, TGF-β1 and IGF-1 in children with Henoch-Schonlein purpura
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摘要: 目的 分析过敏性紫癜(HSP)患儿肠道菌群双歧杆菌与大肠埃希菌数值之比(B/E值)变化与血清低糖基化IgA1(Gd-IgA1)、转化生长因子-β1(TGF-β1)及胰岛素样生长因子-1(IGF-1)的相关性。方法 选择2018年1月至2020年5月在南京鼓楼医院集团宿迁医院儿科住院的128例HSP患儿作为研究对象,根据是否累及肾脏分为普通HSP组73例和紫癜性肾炎(HSPN)组55例。另选取同期健康体检儿童50例作为对照组。采用细菌16S rDNA荧光定量PCR技术检测粪便中双歧杆菌和大肠杆菌数量,并计算B/E值。采用酶联免疫吸附法检测血清低Gd-IgA1、TGF-β1及IGF-1水平。探讨B/E值与血清Gd-IgA1、TGF-β1及IGF-1之间的相关性。结果 HSP组患儿总体肠道双歧杆菌和B/E值均低于对照组,大肠杆菌数量和血清Gd-IgA1、TGF-β1、IGF-1水平均高于对照组(P＜0.05);HSPN组患儿肠道双歧杆菌和B/E值低于普通HSP组,大肠杆菌数量和血清Gd-IgA1、TGF-β1、IGF-1水平高于普通HSP组(P＜0.05);Pearson相关分析显示,HSP患儿肠道菌群B/E值与血清Gd-IgA1、TGF-β1及IGF-1均负相关(r=-0.686、-0.652、-0.598,P＜0.05)。结论 HSP患儿肠道菌群存在肠道菌群失调表现,且HSPN患儿B/E值降低更明显;Gd-IgA1、TGF-β1及IGF-1均参与HSPN的发生发展过程,与肠道菌群失调或具有一定相关性。Abstract: Objective To analyze the correlation between the ratio of bifidobacterium to Escherichia coli (B/E value) and serum galactose-deficient IgA1 (Gd-IGA1), transforming growth factor-β1 (TGF-β1) and insulin-like growth factor-1 (IGF-1) in children with Henoch-Schonlein purpura (HSP). Methods A total of 128 HAP children who were admitted into Department of Pediatrics, Suqian Hospital of Nanjing Gulou Hospital Group from January 2018 to May 2020 were enrolled into the current study. According to the impact on the kidneys, they were divided into two groups: a normal HSP group (n=73) and a Henoch-Schonlein purpura nephritis (HSPN) group (n=55). Meanwhile, another 50 healthy children were selected as a control group. The number of bifidobacterium and Escherichia coli in feces was detected by 16S rDNA fluorescence quantitative PCR, and the B/E value was calculated. The enzyme-linked immunosorbent assay was used to detect the levels of serum galactose-deficient IgA1 (Gd-IGA1), transforming growth factor-β1 (TGF-β1) and insulin-like growth factor-1 (IGF-1). The correlation between B/E value and serum Gd-IGA1, TGF-β1 and IGF-1 was explored. Results Compared with the control group, the HSP group presented remarkable decreases in the number of total intestinal bifidobacterium and B/E value, and increases in the number of Escherichia coli and the levels of serum Gd-IgA1, TGF-β1 and IGF-1 (P<0.05). Compared with the normal HSP group, the HSPN group showed significant decreases in the number of intestinal bifidobacterium and B/E, and the number of Escherichia coli and the levels of serum Gd-IGA1, TGF-β1 and IGF-1 (P<0.05). According to Pearson correlation analysis,t intestinal flora B/E value was significantly negatively correlated with serum Gd-IgA1, TGF-β1 and IGF-1 (r=-0.686, -0.652, -0.598, respectively, P<0.05). Conclusions The intestinal flora of children with HSP was dysregulated, and the decrease of B/E value is more obvious in children with HSPN. Gd-IGA1, TGF-β1 and IGF-1 are all involved in the pathogenesis of HSPN, which may have a certain correlation to intestinal flora dysregulation.
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