Abstract: Objective To investigate the effects of clinical pharmacist intervention on prophylactic application of antibiotics for type Ⅱ incision in hepatopancreatobiliary surgery department. Methods The medical records were collected from patients undergoing type Ⅱ incision surgery from July to November 2019 and 2020. The patients were divided into two groups: a pre-intervention group and a post-intervention group. Both groups were evaluated for the prophylactic use of antibiotics in the perioperative period of type Ⅱ incision surgery. Their selection, timing, and dosage of prophylactic use of antibiotics were compared, while the top five types of antibiotics were observed in the two groups. Results The rate of irrational antibiotics selection fell from 84.20% to 23.92%; the irrational rate of medication replacement fell from 15.35% to 2.29%; the rate of combination use without indication fell from 58.92% to 15.01%; the rate of drug use after surgery decreased from 65.24% to 24.43%; the rate of >2 h preoperative use decreased from 31.38% to 13.74%; the rate of >48 h treatment decreased from 92.1% to 14.25%, and the rate of irrational dose fell 67.04% to 15.52%, and the differences were statistically significant (P<0.05). After intervention, the dosing frequencies of enzyme inhibitors containing preparations and fluoroquinolones decreased, while the use of second-generation cephalosporins increased. ConclusionsPharmaceutical intervention by clinical pharmacists can improve the rationality of prophylactic use of antibiotics for type Ⅱ incision in hepatopancreatobiliary surgery department.