Abstract: Objective To evaluate the value and the relevance of three-dimensional arterial spin labeling imaging (3D ASL) and dynamic susceptibility contrast perfusion weighted imaging (DSC-PWI) in preoperative grading of brain glioma. Methods A total of 39 brain glioma patients were enrolled and their imaging materials were retrospectively analyzed. Their 3D ASL cerebral blood flow (ASL-CBF), DSC cerebral blood flow (DSC-CBF), DSC cerebral blood volume (DSC-CBV) and the relative values were recorded. Results ASL-CBF, DSC-CBF, DSC-CBV and relative values increased with the increase of tumor grade. There was significant positive correlations between ASL-CBF and DSC-CBF, as well as between ASL-rCBF and DSC-rCBF. There was a moderate positive correlation between ASL-CBF and DSC-CBV, as well as between ASL-rCBF and DSC-rCBV. ASL-CBF, DSC-CBF, DSC-CBV and their relative values had a significant positive correlation with their pathological grades. A ROC curve was plotted and the largest AUC was for DSC-rCBE, followed by DSC-rCBV, and ASL-rCBF. Conclusions Both 3D ASL and DSC-PWI quantitative parameters have high sensitivity and specificity in evaluating blood perfusion in glioma, and can be used for preoperative hemodynamic evaluation of glioma. 3D ASL is simple and easy to perform, and can effectively assess the preoperative grading of glioma.