Abstract: Objective To investigate the expression and clinical significance of cyclin D1 (CCND1) and matrix metalloproteinase 9 (MMP9) in ovarian cancer tissues.Methods A total of 58 cases of ovarian cancer tissues collected from patients who underwent surgery in Department of Obstetrics and Gynecology at the Affiliated Hospital of Xuzhou Medical University from October 2019 to December 2021 were included. Meanwhile, 28 cases of normal ovarian tissues collected during the same period were collected. The levels of CCND1 and MMP9 were detected by immunohistochemistry. Clinical pathological data of all patients were collected, and the relationship between the expression of CCND1 and MMP9 and the clinical pathological characteristics of ovarian cancer patients was analyzed. Spearman rank correlation analysis was used to evaluate the correlation of CCND1 and MMP9.Results The positive expression rates of CCND1 and MMP9 in ovarian cancer tissues were significantly higher than those in normal tissues(P<0.05). The expression of CCND1 was related to the FIGO stage of ovarian cancer patients (P<0.05). The expression of MMP9 was related to the histological grade and FIGO stage of ovarian cancer patients (P<0.05). Spearman rank correlation analysis showed a positive correlation between the expression of CCND1 and MMP9 in ovarian cancer tissues (r=0.372, P<0.05).Conclusions CCND1 and MMP9 are upregulated in ovarian cancer tissues, and their expression is associated with the FIGO stage and histological grade of ovarian cancer patients. The expression of both proteins is positively correlated, suggesting a potential interaction and shared involvement in the progression of ovarian cancer.