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    线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性与2型糖尿病患者疲劳的相关性

    Relationship between mitochondrial respiratory chain complex enzyme Ⅰ, Ⅱ, and Ⅲ activities and fatigue in the patients with type 2 diabetic disease

      摘要
    • 摘要: 目的 探讨2型糖尿病（T2DM）患者线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性与疲劳之间的潜在关系。方法 选取2022年1月1日—2022年12月31日于泗洪县第一人民医院就诊的130例T2DM患者作为研究对象，根据疲劳量表评分分为糖尿病无疲乏组和糖尿病疲乏组。采集患者的血样，进行常规的空腹血糖（FBG）、糖化血红蛋白（HbA1c）检测，分别使用辅酶Q10（CoQ10）酶联免疫试剂盒和线粒体呼吸链复合物活性定量检测试剂盒检测CoQ10和呼吸链复合酶Ⅰ、Ⅱ、Ⅲ的活性。采用独立样本t检验、双相关Pearson检验、ROC曲线、二元logistic回归分析评估各项临床指标与糖尿病疲劳风险的相关性。结果 2组患者FBG、HbA1c、24 h平均血糖（24 h MBG）及24 h血糖标准差（24 h SDBG）和简单自测抑郁量表（PHQ-9）、广泛性焦虑量表（GAD-7）评分差异无统计学意义（P>0.05）。疲乏组患者CoQ10和线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ水平均低于无疲乏组患者（P<0.05）。T2DM患者CoQ10水平和线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性与疲劳量表评分呈负相关（r=-0.451～-0.906，P<0.05）。性别（女性）、年龄（60～69岁/70～79岁）、病程（10～年）均为影响T2DM患者产生疲劳感的独立危险因素。结论 线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性降低与T2DM患者疲劳感产生密切相关，而且对T2DM患者疲劳感的产生具有预测价值。

       

      Abstract: Objective To explore the potential relationship between mitochondrial respiratory chain complex enzyme Ⅰ,Ⅱ, and Ⅲ activities and fatigue in type 2 diabetic mellitus (T2DM) patients.Methods A total of 130 T2DM patients who were admitted to the First People's Hospital of Sihong County from January 1, 2020 to December 31, 2021 were selected. According to the Fatigue Scale scores, they were divided into two groups:a T2DM without fatigue group and a T2DM with fatigue group. Their blood samples were collected for routine fasting blood glucose (FBG) test and the glycosylated hemoglobin (HbA1c) test. The levels of coenzyme-Q10 (CoQ10) and the activities of respiratory chain complex enzymes Ⅰ,Ⅱ, and Ⅲ were measured by the Coenzyme Q10 ELISA kit and the Mitochondrial Respiratory Chain Complex Activity Quantitative Kit, respectively. Then, independent samples t-test, double correlation Pearson test, ROC curve, and binary logistic regression analysis were performed to assess the correlation between each clinical index and the risk of diabetic fatigue.Results There were no statistical differences in FBG, HbA1c, mean blood glucose within 24 h (24 h MBG) and 24-hour standard deviation of blood glucose (24 h SDBG), and the scores of the Patient Health Questionnaire-9 item (PHQ-9) and the Generalized Anxiety Disorder Scale-7 item (GAD-7) between the two groups (P>0.05). For patients in the fatigue group, their levels of CoQ10 and mitochondrial respiratory chain complex enzyme Ⅰ,Ⅱ, and Ⅲ were lower than those in the non-fatigue group (P<0.05).For T2DM patients, their CoQ10 and mitochondrial respiratory chain complex enzyme Ⅰ,Ⅱ, and Ⅲ activities were negatively correlated with FS-14 scores (r=-0.451--0.906, P<0.05). Sex (female), age (60-69 years/70-79 years), and disease duration (more than 10 years) were the independent risk factors for fatigue in T2DM patients.Conclusions The decrease in mitochondrial respiratory chain complex enzyme Ⅰ/Ⅱ/Ⅲ activity is closely related to fatigue in T2DM patients. Moreover, they display the predictive value for fatigue in T2DM patients.

       

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