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    淮安地区食源性沙门氏菌耐药性及分子分型研究

    Antimicrobial resistance and molecular subtyping of foodborne Salmonella in Huai′an

    • 摘要: 目的了解分离自食品的沙门氏菌的血清型分布、耐药特性以及分子分型特征。方法对分离自食品的沙门氏菌进行血清凝集分型,利用MIC法测定分离株的耐药性,通过脉冲场凝胶电泳技术对分离株进行基因分型。结果39株分离自食品的沙门氏菌分属于5个群,9种血清型,优势血清型为肠炎沙门氏菌。经脉冲场凝胶电泳分型,按照80%相似度,39株沙门氏菌聚类为15个簇群。39株沙门氏菌中具有耐药性的菌株占 69.23%,耐药率由高到低依次为氨苄西林、萘啶酸、头孢唑啉、庆大霉素、四环素、氯霉素、环丙沙星、甲氧苄氨嘧啶/磺胺甲噁唑、头孢噻肟、头孢他啶和头孢西丁。结论本地区食源性沙门氏菌分离株的血清型和基因型呈多样性分布,并且具有多重耐药的特点。

       

      Abstract: ObjectiveTo elucidate the serotype distribution, antimicrobial resistance and molecular subtyping of Salmonella isolated from foods. MethodsThe serotypes of Salmonella were detected by serum agglutination. Antimicrobial resistance was determined by the minimal inhibitory concentration method and molecular subtyping was carried out by pulsed-field gel electrophoresis. ResultsGenerally, 39 Salmonella isolates were classified 5 groups and 9 serotypes, where the main serotype was Salmonella enteritidis. Meanwhile, 15 PFGE clusters were observed among the 39 isolates with 80% similarity. The percentage of resistant Salmonella was 69.23%, where the resistance rate ranging from high to low was ampicillin, nalidixic acid, cefazolin, gentamicin, tetracycline, chloramphenicol, ciprofloxacin, trimethoprim/sulfamethoxazole, cefotaxime, ceftazidime and cefoxitin. ConclusionSalmonella isolated from foods in this study are phenotypically and genotypically diverse, which are resistant to multiple drugs.

       

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