Abstract: ObjectiveTo explore the effects of rapamycin and chloroquine on the heart of toads after acute alcoholic injury. MethodsThe toads were randomly divided into a rapamycin group (Rap), a chloroquine group (CQ) and a Ringer’s group (RS) (n=15). An acute myocardial injury of toads was established using isolated hearts exposed to 60% alcohol for 2 min followed by being washed with Ringer’s solution. Then, the hearts were infused with 0.1 g/L rapamycin, 0.2 g/L chloroquine or the equal volumes of Ringer’s solution over 20 min before being infused within alcohol. The heart rate and contractility were detected before and after administration. ResultsMyocardial injury induced by alcohol was more obvious at the second time than that at the first time (P<0.05). The heart rate and myocardial contractility revealed an obvious decrease in the chloroquine treatment group compared with the control group (P<0.01), while pretreatment with rapamycin improved the heart rate and myocardial contractility of toads after acute alcoholic injury (P<0.05). ConclusionThe severity of myocardial injury caused by alcohol is correlated to its frequency and infusion. Chloroquine can cause aggravated myocardial damage, while rapamycin improves cardiac function.