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    GOLPH3通过P-YB1途径抑制胶质瘤细胞侵袭和迁移

    GOLPH3 shRNA decreases migration and invasion of human glioma

      摘要
    • 摘要: 目的 探讨癌基因Golgi phosphoprotein 3（G）GOLPH3 shRNA with GFP tagged， 和对照质粒GOLPH3 shNC转入人胶质瘤U25l细胞中，western blot技术检测GOLPH3 ，GOLPH3 L,YB1,P-YB1蛋白表达，细胞划痕试验检测细胞迁移能力，Transwell侵袭实验检测细胞侵袭能力。结果 与对照组比较，转染GOLPH3 shRNA with GFP tagged质粒24 h后U25l细胞侵袭能力下降49％、迁移能力下降58％，差异均有统计学意义(P<0.01)；转染GOLPH3 shRNA 可以明显降低YB1,P-YB1蛋白的表达。结论 下调GOLPH3可以通过降低YB1,P-YB1的表达，进而抑制胶质瘤细胞侵袭和迁移能力。

       

      Abstract: objective To investigate the effect and mechanism of GOLPH3 on the human glioma cells migration and invasion．Methods Transfection of the GOLPH3 shRNA with GFP tagged plasmid into the U25l glioma cell were carried out using Lipofectamine 2000．Wound healing assay and Transwell invasion assay were used to analyze the influence on the migration and invasion of glioma U251 cells after transfection of the GOLPH3 shRNA with GFP tagged plasmid. Western blot was used to analyze the protein expressions of YB1/ P-YB1 after GOLPH3 down-regulation . Results Compared to the control plasmids, GOLPH3 shRNA with GFP tagged plasmid could decrease the expression of YB1/ P-YB1 in glioma U251 cells . In cell invasion assay， GOLPH3 shRNA inhibits cell invasion ability by 49％, In cell migration assay，GOLPH3 shRNA inhibits cell migration ability by 58％; Down-regulation of GOLPH3 decreased the levels of YB-1 and pYB-1S102 in glioma U251 cells. Conclusion Down-regulation of GOLPH3 may be inhibit glioma cells migration and invasion via decreasing the expression of YB1/ P-YB1

       

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