Abstract: Objective：To investigate the nutritional state of vitamin A and E in obese children, and to analysis the relationship between vitamin A and E and obesity. Methods：Fifty overweight or obese children and Fifty healthy children were divided into overweight group, obese group and control group. The venous blood was collected to detect the content of vitamin A and E. Results: There was statistical difference in serum level of vitamin A and E among three groups (P<0.05). Serum levels of vitamin A in overweight and obesity children were significantly lower than that in the controls (P<0.05). The level of vitamin E in obese group was significantly decreased compared with control group (P<0.05). The insufficiency rate of vitamin E was obviously different and showed the trend of obese group>overweight group> control group decreased. Serum levels of vitamin A and E were inversely associated with BMI (r= -0.395，P=0.000；r= - 0.374，P=0.000). Conclusions: Serum levels of vitamin A and E in overweight and obese children were significantly decreased，which maybe associated with the occurrence of obesity in pre-school children