Abstract: Objective To evaluate the diagnostic value of serumglutamyl transpeptadase (GGT) and glycocholic acid(CG) in intrahepatic cholestasis of pregnancy (ICP).Methods Retrospective analysis was performed on 157 ICP patients who were admitted to Department of Obstetrics and Gynecology and Department of Gastroenterology, Xuzhou Central Hospital from January 2017 to June 2022. and set as an ICP group. Meanwhile, another 119 normal pregnant women who were admitted to Xuzhou Central Hospital were set as a control group. Their general clinical data and laboratory indicators were compared, while the risk factors of ICP were analyzed by logistic regression. The diagnostic value of serum GGT and CG in ICP was evaluated through receiver operating characteristic (ROC) analysis.Results There were statistical differences in maternal age, gestational age, total bilirubin (TBil), international standardized ratio (INR), alkaline phosphatase (ALP), aspartate aminotransferase (AST), GGT, total bile acid (TBA) and CG between the ICP group and the control group (P<0.05). According to logistic regression analysis, GGT (Exp(B)=1.006, 95%CI=1.002, 1.010, P=0.004) and CG (Exp(B)=1.046, 95%CI=1.032, 1.061, P<0.001) were positively correlated with the occurrence of ICP. ROC analysis indicated that the AUC of GGT in diagnosing ICP was 0.85 (95%CI=0.801, 0.901, P<0.001), with a sensitivity of 82.17% and a specificity of 91.60%, and the Youden index was 73.77. The AUC of CG in diagnosing ICP was 0.95 (95%CI=0.932, 0.975, P<0.001), with a sensitivity of 90.45% and a specificity of 87.39%, and the Youden index was 77.84. The AUC of GGT in combination with CG in diagnosing ICP was 0.98 (95%CI=0.966, 0.992, P<0.001), with a sensitivity of 93.28% (95%CI=87.180, 97.050) and a specificity of 94.74% (95%CI=89.890, 97.700), and the Youden index was 88.02.Conclusions The levels of serum GGT and CG remarkably increase in ICP patients, and are related to the occurrence of ICP. Detection of both indicators can improve the diagnostic efficiency of ICP.