Detection of pathogen characteristics of lower respiratory tract infection patients and lung cancer patients with lower respiratory tract infection by LAMP assay

Objective To analyze the characteristics of sputum pathogens in lower respiratory tract infection patients and lung cancer patients with lower respiratory tract infection, and clarify the pathogens of lower respiratory tract infections, so as to improve clinical effectiveness. Methods A total of 630 sputum samples from patients who were admitted in Xuzhou Central Hospital due to lower respiratory tract infection were collected. They were detected by a commercially available loop-mediated isothermal amplification (LAMP) assay kit to analyze pathogen characteristics. Results the sputum samples of lower respiratory tract infection patients, the two most frequently detected pathogens were Klebsiella Pneumoniae (Kpn) and Haemophilus influenza (Hin). Compared with lower respiratory tract infection patients without tumor and basic diseases, lung cancer patients with lower respiratory tract infection presented a higher positive rate of Hin (P<0.05). The consistent rate between the LAMP method and the culture method was 68.7%. Conclusions Low respiratory tract infection patients present a high positive detection rate of pathogens through LAMP method, with a very high positive rate of Hin in lung cancer patients with low respiratory tract infection. These results provide important evidence for personalized treatment of lower respiratory tract infection patients and lung cancer patients with lower respiratory tract infection.