Effects of KIFC1 gene on the proliferation and metastasis of colorectal cancer

Objective To investigate the effects of KIFC1 gene on the proliferation and metastasis of colorectal cancer. Methods Western blotting was used to detect the expression of KIFC1 in colorectal cancer and para-carcinoma tissues. Human colorectal cancer cells were transfected with KIFC1-siRNA, and the expression of KIFC1 was detected by Western blotting. CCK-8 assay was used to detect the effect of KIFC1 gene on the proliferation of colorectal cancer cells. Transwell assay was conducted to detect the effect of KIFC1 gene on the migration of colorectal cancer cells. Western blotting was used to detect the expression of EMT-related proteins, such as E-cadherin, N-cadherin and Vimentin. Results GEPIA database and immunoblotting results showed that KIFC1 was highly expressed in colorectal cancer tissue. Compared with the control group, KIFC1-siRNA could significantly reduce the expression of KIFC1 protein in colorectal cancer cells. CCK-8 assay showed that KIFC1-siRNA significantly inhibited the proliferation of colorectal cancer cells. Transwell assay showed that KIFC1-siRNA significantly reduced the metastatic ability of colorectal cancer cells, while inhibiting the expression of KIFC1 so as to stimulate the expression of E-cadherin protein and inhibit the expression of N-cadherin and Vimentin protein (P<0.05). Conclusions Inhibition of the expression of KIFC1 can inhibit the proliferation and metastasis of colorectal cancer cells.