Expression of SH3BGR gene in cervical cancer tissues and its clinical significance

Objective To analyze the expression of SH3 domain binding glutamate rich protein (SH3BGR) gene in cervical cancer tissues and its clinical significance and explore the effects of SH3BGR on the malignancy, proliferation, and migration of HeLa cells and related mechanisms. Methods Immunohistochemistry (IHC) was used to detect the expression of SH3BGR protein in cervical cancer tissues. TCGA database and bio-information methods were used to analyze the relationship between the expression of SH3BGR, and the clinical stage of cervical cancer and patient survival. SH3BGR gene was amplified by polymerase chain reaction (PCR) and cloned into a pHAGE-CMV-MCS-IzsGreen vector to construct a plasmid of recombinant lentiviral vector pHAGE-SH3BGR. The recombinant plasmid pHAGE-SH3BGR, the envelope plasmid pMD2.G and the packaging plasmid psPAX2 were co-transfected into human embryonic kidney epithelial 293 T cells. The virus titer was determined by the gradient dilution method. After infection of the recombinant lentivirus SH3BGR into HeLa cells, the expression of SH3BGR was measured by Western blot. Then, the CCK-8 assay, wound-healing assay and soft agar assay were adopted to evaluate the effects of overexpression of SH3BGR on the proliferation, migration and malignancy of HeLa cells. The small interfering RNA (siRNA) method was used to knock down the gene of human papillomavirus (HPV) E6/E7, before measurement of the expression of SH3BGR protein. Results The expression of SH3BGR protein in cervical cancer tissues was significantly higher than that in the normal adjacent tissues. The survival time of cervical cancer patients with higher SH3BGR expression was significantly shorter than those with low SH3BGR expression. Overexpression of SH3BGR protein remarkably promoted the proliferation, migration and malignancy of HeLa cells. Knocking down of HPV E6/E7 inhibited the expression of SH3BGR protein. Conclusions HPV may up-regulate the expression of SH3BGR via E6/E7 protein, so as to promote the malignancy, proliferation and migration of HeLa cells. High expression of SH3BGR protein is not beneficial to the survival of cervical cancer patients.