Preparation of hydrogen sulfide sustained-release gel and its effects on skin ulcer in diabetic mice

Objective To prepare delayed-release hydrogen sulfide (H₂S) gel and evaluate its effects on the healing of skin ulcer wound in diabetic mice. Methods β-Cyclodextrin-coated diallyl trithioether (DATS@CD) was dissolved in alginate gel, and glutathione (GSH) was dissolved in calcium chloride gel with chitosan (Cs). The resultant substance was used to treat skin ulcer wound in diabetic mice. Results DATS reacted with GSH and CD to produce H₂S, and effectively slowed the rate of reaction, while stimulating the healing of skin ulcer in diabetic mice. Conclusions Delayed-release H₂S gel is successfully prepared, which can effectively promote the healing of skin ulcer in diabetic mice.