Advanced Search
HU Ping, LU Junjie, GUO Aisong. The expression and clinical significance of ASPHD1 in colorectal cancer[J]. Journal of Xuzhou Medical University, 2021, 41(7): 499-503. DOI: 10.3969/j.issn.2096-3882.2021.07.006
Citation: HU Ping, LU Junjie, GUO Aisong. The expression and clinical significance of ASPHD1 in colorectal cancer[J]. Journal of Xuzhou Medical University, 2021, 41(7): 499-503. DOI: 10.3969/j.issn.2096-3882.2021.07.006

The expression and clinical significance of ASPHD1 in colorectal cancer

More Information
  • Received Date: August 25, 2020
  • Revised Date: June 15, 2021
  • Objective To determine the genes with high sensitivity and specificity in colorectal cancer based on bioinformatics and molecular biological experiments. Methods Bioinformatics software was used to analyze the highly expressed biomarker aspartate beta-hydroxylase domain-containing protein 1 (ASPHD1) in colorectal cancer tissues. The expression of ASPHD1 in colorectal cancer cell lines and its effects on cell viability were measured by real-time fluorescence quantitative PCR and CCK-8 assay, respectively. Results The levels of ASPHD1 in 275 samples of colon adenocarcinoma, 349 samples of normal colon, 92 samples of rectal adenocarcinoma and 318 samples of normal rectal tissue were investigate using GEPIA database. Compared with normal tissues, the expression of ASPHD1 in colorectal cancer tissues significantly increased. The overall survival rate of colon adenocarcinoma patients with high expression of ASPHD1 was significantly lower than those with low expression of ASPHD1 (P<0.05). ASPHD1 was associated with clinical stage, and lymph node and distant metastasis (P<0.05). ASPHD1 was positively correlated with the expression of Vimentin encoding gene Vim, a marker of epithelial-mesenchymal transition (R=0.11, P<0.05). The results of real-time fluorescence quantitative PCR showed that the levels of ASPHD1 in intestinal cancer cell lines SW480, SW620, HCT116 and LoVo increased compared with those in human normal intestinal epithelial cells NCM460. Small interfering RNA of ASPHD1 significantly inhibited the cell viability of HCT116 and LoVo. Conclusions ASPHD1 is highly expressed in colorectal cancer tissues, which is related to the prognosis of colorectal cancer and affects the cell viability of intestinal cancer cells. It can be used as a biomarker for colorectal cancer and provide new thoughts for clinical treatment.
  • [1]
    Siegel RL,Miller KD,Jemal A.Cancer statistics,2020[J].CACancer J Clin,2020,70(1):7-30.
    [2]
    Tauriello DV,Calon A,Lonardo E,et al.Determinants of metastatic competency in colorectal cancer[J].Mol Oncol,2017,11(1):97-119.
    [3]
    Tang Z,Li C,Kang B,et al.GEPIA:a web server for cancer and normal gene expression profiling and interactive analyses[J].Nucleic Acids Res,2017,45(w1):W98-W102.
    [4]
    Chandrashekar DS,Bashel B,Balasubramanya SAH,et al.UALCAN:a portal for facilitating tumor subgroup gene expression and survival analyses[J].Neoplasia,2017,19(8):649-658.
    [5]
    Sepulveda AR,Hamilton SR,Allegra CJ,et al.Molecular biomarkers for the evaluation of colorectal cancer:guideline summary from the American society for clinical pathology,college of American pathologists,association for molecular pathology,and American society of clinical oncology[J].J Oncol Pract,2017,13(5):333-337.
    [6]
    Yang Z,Chen Y,Wu D,et al.Analysis of risk factors for colon cancer progression[J].Onco Targets Ther,2019,12:3991-4000.
    [7]
    Jing C,Wang T,Ma R,et al.New genetic variations discovered in kras wild-type cetuximab resistant chinese colorectal cancer patients [J].MolCarcinog,2020,59(5):478-491.
    [8]
    Oh BY,Kim SY,Lee YS,et al.Twist1-induced epithelial-mesenchymal transition according to microsatellite instability status in colon cancer cells[J].Oncotarget,2016,7(35):57066-57076.
    [9]
    Tang J,Li Y,Wang J,et al.Molecular mechanisms of microRNAs in regulating epithelial-mesenchymal transitions in human cancers[J].Cancer Lett,2016,371(2):301-313.
    [10]
    Lavenus SB,Tudor SM,Ullo MF,et al.A flexible network of vimentin intermediate filaments promotes migration of amoeboid cancer cells through confined environments[J].J Biol Chem,2020,295(19):6700-6709.
    [11]
    Du L,Li JC,Lei L,et al.High vimentin expression predicts a poor prognosis and progression in colorectal cancer:a study with meta-analysis and TCGA database[J].Biomed Res Int,2018,2018:6387810.
    [12]
    Toiyama Y,Yasuda H,Saigusa S,et al.Increased expression ofSlug and Vimentin as novel predictive biomarkers for lymph node metastasis and poor prognosis in colorectal cancer[J].Carcinogenesis,2013,34(11):2548-2557.

Catalog

    Article views (789) PDF downloads (661) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return