Antitumor effects of interfering adenovirus targeting LASP1 gene on non-small cell lung cancer

Objective To investigate the antitumor effect of interfering adenovirus targeting LIM and SH3 protein 1 (LASP1) gene (Ad-LASP1-shRNA) on non-small cell lung cancer (NSCLC). Methods Real-time fluorescence quantitative polymerase chain reaction (qPCR) and Western blot were used to detect the levels of LASP1 mRNA and protein in NSCLC tissue. Then, A549 cells were transfected with Ad-LASP1-shRNA for 48 h. The expression of LASP1 mRNA and protein in the transfected A549 cells were analyzed by qPCR and Western blot. The wound healing and Transwell assay were used to detect the migration and invasion ability. A xenograft model was established using A549 cells. Then, Ad-LASP1-shRNA was intra-tumorally injected and tumor volume was measured regularly. Results NSCLC tissue and normal adjacent tissue showed statistical different relative amounts of LASP1 mRNA and protein (P<0.001). According to qPCR and Western blot, Ad-LASP1-shRNA effectively inhibited the expression of LASP1. After transfection with Ad-LASP1-shRNA for 24 h, the A549 cells presented remarkable increases in the abilities of migration and invasion, and the number of invasive and migratory cells, compared with the control group (P<0.05). After implantation for five weeks, the Ad-LASP1-shRNA group showed a tumor volume of (334.5±32.6) mm³, which were significantly smaller than that of the PBS group (833.9±61.3) mm³ (P<0.001). Ad-LASP1-shRNA was able to significantly inhibit the growth of xenograft tumor. Conclusions Ad-LASP1-shRNA shows obvious anti-tumor effects and underlying mechanisms needed further investigation.