Effects of Nr-CWS on the treatment of diabetic wounds by enhancing the secretion function of ADSCs

Objective To explore the effects of Nocardia rubra cell wall skeleton (Nr-CWS) on diabetic wounds by enhancing the secretion function of adipose derived stem cells (ADSCs). Methods ADSCs were prepared in vitro and identified. Then, the conditional medium of ADSCs (ADSC-CM) and those stimulated by Nr-CWS (ADSC-CM^Nr-CWS) were prepared. Human umbilical vein endothelial cells (HUVECs) were divided into three groups: a high-glucose control group, an ADSC-CM group and an ADSC-CM^Nr-CWS group. The contents of cytokines in ADSC-CM were detected by ELISA. The proliferation, invasion, migration and angiogenesis of HUVECs were measured by CCK-8 assay, Transwell assay, wound healing test and angiogenesis test, respectively. For in vivo experiments, a diabetic wound model of mice was established. ADSC -CM^Nr-CWS were injected around the wound surface, and the speed and quality of wound healing and wound angiogenesis were analyzed. Results Compared with the high-glucose control group and ADSC-CM group, the ADSC-CM^Nr-CWS group released increased amounts of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and hematopoietic growth factor (HGF) (P＜0.05), and HUVECs in the ADSC -CM^Nr-CWS group presented remarkable improvement in the proliferation, invasion, migration and angiogenesis (P＜0.05). For in vivo studies, compared with the high-glucose control group and ADSC-CM group, the ADSC-CM^Nr-CWS group showed obvious increases in wound angiogenesis and wound healing rate, and the quality of wound repair was also significantly improved(P＜0.05). Conclusions Nr-CWS can promote the angiogenesis of diabetic wounds and wound healing by improving the secretion function of ADSCs.