Expression and clinical significance of miR-182-5p and TRIM8 in endometrial cancer

Objective To investigate the expression of miRNA-182-5p (miR-182-5p) and three-domain protein 8 (TRIM8) in endometrial cancer and its clinical significance. Methods A total of 76 patients with endometrial cancer who underwent surgical treatment in Xuzhou Central Hospital from January 2015 to June 2016 were selected. The surgically removed endometrial cancer tissues and adjacent normal tissues were collected. The expression of TRIM8 protein in the tissues was detected by immunohistochemistry. The levels of miR-182-5p and TRIM8 mRNA were detected by qRT-PCR. The correlation between miR-182-5p and TRIM8 was analyzed by Spearman method. The prognostic factors of endometrial cancer were analyzed by Kaplan-Meier method and Cox proportional risk regression model. Results Immunohistochemical results showed that the positive expression rate of TRIM8 in endometrial cancer tissues (55.26%) was lower than that in adjacent normal tissues (73.68%) (P<0.05). qRT-PCR results showed that endometrial cancer tissues showed increased miR-182-5p levels and decreased TRIM8 levels, compared with adjacent normal tissues (P<0.05).The expression of miR-182-5p and TRIM8 in endometrial cancer tissues was related with FIGO stage and lymph node metastasis (P<0.05). Spearman correlation analysis showed that miR-182-5p was negatively related with TRIM8 expression (r=-0.575, P<0.05). Kaplan-Meier analysis showed that patients with high miR-182-5P expression and low TRIM8 expression had a poor prognosis (P<0.05). Cox multivariate regression analysis indicated that miR-182-5p and TRIM8 were the independent risk factors influencing the prognosis of patients with endometrial cancer. Conclusions The expression of miR-182-5p is up-regulated and the expression of TRIM8 is down-regulated in endometrial cancer tissue. The dysregulation of miR-182-5p and TRIM8 may be involved in the pathogenesis of endometrial cancer.