Effects of ADRM1 on the proliferation and metastasis of pancreatic adenocarcinoma cells and underlying mechanism

Objective To investigate the effect of adhesion regulating molecule 1 (ADRM1) on the proliferation and metastasis of pancreatic adenocarcinoma cells and underlying mechanism. Methods GEPIA database was used to analyze the level of ADRM1 mRNA in pancreatic adenocarcinoma and its effect on clinical prognosis. The effects of silencing or overexpression of ADRM1 on the proliferation and metastasis of pancreatic cancer cells were analyzed by EdU, CCK-8 and Transwell assays. Bioinformation assay and Western blot were used to investigate the mechanism of ADRM1 in regulating the proliferation and metastasis of pancreatic cancer cells. Results The level of ADRM1 mRNA was up-regulated in pancreatic cancer, which was not correlated with overall survival, but was negatively correlated with disease-free survival. The high ADRM1 mRNA group showed lower disease-free survival than the low ADRM1 mRNA group.Inhibiting ADRM1 resulted in decreases in the proliferation and metastasis capacities of pancreatic cancer cells, while overexpression of ADRM1 enhanced the proliferation and metastasis of pancreatic cancer cells. ADRM1 activated the JAK tyrosine protein kinase 2-signal transduction and transcription activator 3 (JAK2-STAT3) signaling pathway. Conclusions As an oncogene, ADRM1 is up-regulated in pancreatic adenocarcinoma and promotes the proliferation and metastasis of cells, which may be related to activation of the JAK2-STAT3 signaling pathway.