Comparison of the clinical diagnostic value of CA125,HE4 and mSEPT9 in endometrial cancer

Objective To evaluate serum human epididymal protein 4(HE4), carbohydrate antigen 125(CA125) and methylation of SEPT9(mSEPT9) in the urine in the diagnosis of endometrial cancer.Methods A total of 40 patients who were admitted to the Affiliated Hospital of Xuzhou Medical University and diagnosed with endometrial cancer were selected as a research group. Meanwhile, 39 patients who were diagnosed with benign endometrial lesions were selected as a control group. Their levels of serum HE4 and CA125 were detected by chemiluminescence. Their Ct values of mSEPT9 in the urine were detected by real-time fluorescent quantitative PCR.Results The research group showed remarkably higher levels of serum CA125 and HE4 than the control group(P<0.05). According to ROC curves, the cut-off values of serum CA125, HE4 and urine mSEPT9 Ct values were 14.65 kU/L, 52.44 pmol/L and 38.95, respectively. The research group presented significantly higher detection rates of serum CA125 and HE4 and urine mSEPT9 than the control group(P<0.05). According to ROC curves, the area under the curve of mSEPT9, CA125 and HE4 were all >0.5, with clinical diagnostic significance. The specificity of mSEPT9 was higher than those of HE4 and CA125. Furthermore, CA125 level was correlated with pathological stage, pathological grade and lymphatic vascular metastasis(P<0.05); HE4 level was correlated with age and depth of muscle infiltration(P<0.05); and the positive rate of mSEPT9 was correlated with pathological stage, depth of muscle infiltration, lymphatic and vascular metastasis(P<0.05).Conclusions Detection of urine mSEPT9 has great potential in the screening of endometrial cancer, prociding new thoughts for endometrial cancer screening.