Expression of miR-146b-5p and TRIM29 in endometrial carcinoma

MA Chenglei; ZHANG Yajie; TONG Jianye; YAN Hongchao

doi:10.3969/j.issn.2096-3882.2023.01.011

Journal of Xuzhou Medical University > 2023 > 43(1): 59-64. > DOI: 10.3969/j.issn.2096-3882.2023.01.011

MA Chenglei, ZHANG Yajie, TONG Jianye, YAN Hongchao. Expression of miR-146b-5p and TRIM29 in endometrial carcinoma[J]. Journal of Xuzhou Medical University, 2023, 43(1): 59-64. DOI: 10.3969/j.issn.2096-3882.2023.01.011

Citation:

PDF (1388 KB)

Expression of miR-146b-5p and TRIM29 in endometrial carcinoma

1. Department of Obstetrics and Gynecology, Graduate School, Xuzhou Medical University, Xuzhou 221004, China;
2. Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221006;
3. Department of Obstetrics and Gynecology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002

More Information

Received Date: November 15, 2022
Revised Date: January 07, 2023
Available Online: January 01, 2024

Graphical Abstract

Abstract

Abstract

Objective To investigate the expression of miRNA-146b-5p(miR-146b-5p) and tripartite motif protein 29(TRIM29) in endometrial cancer, and its clinical significance.Methods A total of 60 patients with endometrial cancer and 30 patients with normal proliferative endometrium were selected from the Affiliated Hospital of Xuzhou Medical University and the Second Affiliated Hospital of Xuzhou Medical University from October 2019 to October 2020. Their endometrial tissues were collected to detect the expression of TRIM29 by immunohistochemistry. The expression of miR-146b-5p and TRIM29 mRNA was measured by qRT-PCR. The correlation between the expression of miR-146b-5p and TRIM29 was evaluated by Spearman analysis. Then, Kaplan-Meier method and Cox proportional risk regression model were used to analyze the prognosis.Results According to immunohistochemical results, the positive expression rate of TRIM29 in endometrial carcinoma tissues(55.0%) was lower than that in normal proliferative endometrial tissues(76.7%)(P<0.05). Then, qRT-PCR results showed that the expression of miR-146b-5p in endometrial cancer tissues was significantly higher than that in normal proliferative endometrial tissues, while the expression of TRIM29 was significantly lower than that in normal proliferative endometrial tissues(P<0.05). Spearman analysis indicated that the expression of miR-146b-5p was negatively correlated with TRIM29(r=-0.595, P<0.05). Kaplan-Meier analysis showed that patients with high expression of miR-146b-5p and low expression of TRIM29 had poor prognosis(P<0.05). Cox multivariate regression analysis demonstrated that miR-146b-5p and TRIM29 were the independent factors affecting the prognosis of patients with endometrial cancer.Conclusions High expression of miR-146b-5p and low expression of TRIM29 in endometrial cancer tissues may be involved in the pathogenesis of endometrial cancer.
- endometrial cancer,
- miR-146b-5p,
- TRIM29,
- prognosis

FullText(HTML)

References (18)

References

[1]	Lu KH,Broaddus RR.Endometrial cancer.reply[J].N Engl J Med,2021,384(6):586.
[2]	Straubhar AM,Parsons MW,Francis S,et al.Refusal of surgery and survival outcomes in endometrial cancer[J].Int J Gynecol Cancer,2021,31(9):1236-1241.
[3]	Lortet-Tieulent J,Ferlay J,Bray F,et al.Internationalpatterns and trends in endometrial cancer incidence,1978-2013[J].J Natl Cancer Inst,2018,110(4):354-361.
[4]	Bartel DP.Metazoan microRNAs[J].Cell,2018,173(1):20-51.
[5]	Tan WG,Liu BD,Qu SH,et al.MicroRNAs and cancer:key paradigms in molecular therapy[J].Oncol Lett,2018,15(3):2735-2742.
[6]	Li YW,Zhang HB,Dong YL,et al.MiR-146b-5p functions as a suppressor miRNA and prognosis predictor in non-small cell lung cancer[J].J Cancer,2017,8(9):1704-1716.
[7]	Jiang K,Li GP,Chen WJ,et al.Plasma exosomal miR-146b-5p and miR-222-3p are potential biomarkers for lymph node metastasis in papillary thyroid carcinomas[J].Onco Targets Ther,2020,13:1311-1319.
[8]	Santa-Inez DC,Fuziwara CS,Saito KC,et al.Targeting the highly expressed microRNA miR-146b with CRISPR/Cas9n gene editing system in thyroid cancer[J].Int J Mol Sci,2021,22(15):7992.
[9]	Huang XH,Zhong R,He XK,et al.Investigations on the mechanism of progesterone in inhibiting endometrial cancer cell cycle and viability via regulation of long noncoding RNA NEAT1/microRNA-146b-5p mediated Wnt/β-catenin signaling[J].IUBMB Life,2019,71(2):223-234.
[10]	Luo SX,Shen M,Chen H,et al.Long non-coding RNA TP73-AS1 accelerates the progression and cisplatin resistance of non-small cell lung cancer by upregulating the expression of TRIM29 via competitively targeting microRNA-34a-5p[J].Mol Med Rep,2020,22(5):3822-3832.
[11]	Hatakeyama S.Early evidence for the role of TRIM29 in multiple cancer models[J].Expert Opin Ther Targets,2016,20(7):767-770.
[12]	Yin XD,Zhang J,Li CJ,et al.LncRNA HOXA11-AS accumulation-induced microRNA-761 downregulation regulates cell growth by targeting TRIM29 in papillary thyroid cancer[J].Am J Transl Res,2019,11(11):6826-6837.
[13]	Xu MX,Hu JX,Zhou BX,et al.TRIM29 prevents hepatocellular carcinoma progression by inhibiting Wnt/β-catenin signaling pathway[J].Acta Biochim Biophys Sin (Shanghai),2019,51(1):68-77.
[14]	Qiao HY,Zhang Q,Wang JM,et al.TRIM29 regulates the SETBP1/SET/PP2A axis via transcription factor VEZF₁ to promote progression of ovarian cancer[J].Cancer Lett,2022,529:85-99.
[15]	Gentry-Maharaj A,Karpinskyj C.Current and future approaches to screening for endometrial cancer[J].Best Pract Res Clin Obstet Gynaecol,2020,65:79-97.
[16]	Qiu ZW,Li H,Wang JW,et al.MiR-146a and miR-146b in the diagnosis and prognosis of papillary thyroid carcinoma[J].Oncol Rep,2017,38(5):2735-2740.
[17]	Xu JK,Li ZY,Su QH,et al.Suppression of long noncoding RNA LINC00324 restricts cell proliferation and invasion of papillary thyroid carcinoma through downregulation of TRIM29 via upregulating microRNA-195-5p[J].Aging (Albany NY),2020,12(24):26000-26011.
[18]	Zhang ZM,Fu XL,Gao YY,et al.LINC01535 attenuates ccRCC progression through regulation of the miR-146b-5p/TRIM2 axis and inactivation of the PI3K/Akt pathway[J/OL].J Oncol,2022,2022:2153337.

Cited By

Get Citation

PDF

XML

Article views (195) PDF downloads (187)

Turn off MathJax

Article Contents

Abstract

References

Expression of miR-146b-5p and TRIM29 in endometrial carcinoma

Abstract

References

Catalog

Export File

Citation

Format

Content