Expression and clinical significance of E2F7 in gastric carcinoma tissues

ObjectiveTo explore the expression and clinical significance of E2F7 in gastric carcinoma tissues and analyze the relationship between the levels of E2F7 and prognosis of gastric carcinoma. MethodsA total of 45 samples of gastric cancer tissues and adjacent non-cancerous tissues were randomly collected and the level of E2F7 mRNA was detected by quantitative real-time PCR. The level of E2F7 protein in 93 samples of gastric cancer and adjacent tissues was detected by immunohistochemistry. The association among E2F7 level with the clinical data and prognosis of the gastric cancer patients was analyzed. ResultsAccording to real-time PCR,the level of E2F7 mRNA in gastric cancer tissues was remarkably higher than that in adjacent tissues (P<0.01). The positive expression rate of E2F7 protein was significantly higher in gastric cancer tissues than that in adjacent tissues (P<0.01). The expression of E2F7protein was associated with tumor infiltration depth, lymph node metastasis and TNM stage (P<0.01), rather than age, gender, tumor location, differentiation degree and tumor size.Kaplan-Meier analysis indicated no significant difference in survival time after surgery between gastric cancer patients with high and low E2F7 protein expression (P>0.05). ConclusionThe levels of E2F7 mRNA and protein in gastric cancer tissues are up-regulated and associated with tumor infiltration depth,lymph node metastasis and TNM stage, which can be further investigated in the future