Effects of neostigmine on incision healing in mice

ObjectiveTo observe the effects of different doses of intraperitoneal injection of neostigmine on incision healing in mice. MethodsKunming mice were randomly divided into four groups (n=10): a normal saline (NS) group, and neostigmine 17.5 μg·kg-1, 35 μg·kg-1, 70 μg·kg-1 (N17.5、N35、N70) groups. Corresponding doses of neostigmine were intraperitoneally injected in the N17.5, N35, and N70 groups 10 min before skin incising, while the same volume of NS was administered in the NS group. The incision length and weight increase were measured 72 h after operation. The average incision healing time in each group was recorded and the daily cumulative incision healing rate was calculated. ResultsCompared with the NS group, the incision length was shortened in the N35 and N70 groups 72 h after the operation (P<0.05 or P<0.01). The incision length was shortened more obviously in the N17.5 and N35 groups than that in the N70 group (P<0.01). The average incision healing time in the N17.5, N35 and N70 groups were decreased, compared with the NS group (P<0.05). Meanwhile, the daily cumulative incision healing rate in the N17.5, N35 and N70 groups was higher than that in the NS group, where the N70 group showed the highest healing rate. ConclusionsNeostigmine at 35 and 70 μg·kg-1 can promote incision healing in mice, while no obvious improvement was observed in mice receiving 17.5 μg·kg-1 neostigmine.