Antimicrobial resistance and molecular subtyping of foodborne Salmonella in Huai′an

ObjectiveTo elucidate the serotype distribution, antimicrobial resistance and molecular subtyping of Salmonella isolated from foods. MethodsThe serotypes of Salmonella were detected by serum agglutination. Antimicrobial resistance was determined by the minimal inhibitory concentration method and molecular subtyping was carried out by pulsed-field gel electrophoresis. ResultsGenerally, 39 Salmonella isolates were classified 5 groups and 9 serotypes, where the main serotype was Salmonella enteritidis. Meanwhile, 15 PFGE clusters were observed among the 39 isolates with 80% similarity. The percentage of resistant Salmonella was 69.23%, where the resistance rate ranging from high to low was ampicillin, nalidixic acid, cefazolin, gentamicin, tetracycline, chloramphenicol, ciprofloxacin, trimethoprim/sulfamethoxazole, cefotaxime, ceftazidime and cefoxitin. ConclusionSalmonella isolated from foods in this study are phenotypically and genotypically diverse, which are resistant to multiple drugs.