Target genes prediction and bioinformatics analysis of hsa-miR-199b-5p

Objective: To identify the potential biological function of hsa-miR-199b-5p. Methods：First and foremost, we used miRbase and UCSC databases to analyzed its conservation of the sequence. Secondly, we predicted the target genes of hsa-miR-199b-5p through Miranda, miRDB and TargetScan databases. Then we get the intersection of these predicted genes . In the end, by using GO and KEGG pathway analysis, we clarified the cell functions and signaling pathways which are regulated by hsa-miR-199b-5p target genes . Results: Given the bio-informatics data, we figured out that hsa-miR-199b-5p may have a wide range of functions. Also, some potential target genes of hsa-miR-199b-5p might be involved in the initiation and development of podocyte injury. Conclusion: Hsa-miR-199b-5p might be related to the pathogenesis of CKD. Thus,it might provide the clinical therapy with a potential target to CKD